2. From the InstaGene matrix manufacture\'s website \"InstaGene matrix, made wit

Question

2. From the InstaGene matrix manufacture's website "InstaGene matrix, made with a specially formulated 6% w/v Chelex resin, makes DNA sample preparation fast, easy, and cost-effective, providing PCR-quality template DNA in less than an hour. The Chelex matrix binds to PCR inhibitors rather than DNA, preventing DNA loss due to irreversible DNA binding. The InstaGene matrix eliminates the time-consuming and labor-intensive deproteinization, organic extraction, dialysis, and alcohol precipitation protocols required in traditional DNA purification procedures. Place the cells in a microcentrifuge tube, add the InstaGene matrix, boil, and spin. The matrix adsorbs cell lysis products that interfere with the PCR amplification process. DNA in the supernatant is ready for PCR." Why does our protocol include a step for grinding the plant material in a mortar and pestle before adding the plant cells to a tube containing InstaGene matrix? (Hint: What is something that makes plant cells distinct from many other cell types?)

Explanation / Answer

The purpose of the grinding mechanism is two fold:

1) to disrupt the tissues and membranes (leaf sheath) into wich the cells are arranged

2) to disintegrate the tough cellulose present in the cell walls of the plants which are absent from the animal cells; this damages and opens the cells to allow easy DNA isolation.

Related Questions

Navigate

• Browse (All)
• Browse 2
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.