The mRNA sequence below contains nucleotides that encode the first 5 amino acids

Question

The mRNA sequence below contains nucleotides that encode the first 5 amino acids of the protein made by the nuclear fallout (nuf) gene in Drosophila: 5'-GUAAAUCAUGAGUAGUCAACCUGCCAGC...-3' wildtype mRNA What are the first five amino acids encoded by this mRNA (use codon table on last page)? You isolate a mutant nuf gene and determine the base sequence of the mutant mRNA: 5'-GUAAAUCAUGAGUAGUUAACCUGCCAGC...-3' mutant mRNA What is the new amino acid sequence? Is the mutation in the gene a transition, transversion, insertion, or deletion? Is the mutation a missence or a nonsence mutation? Match the type of RNA with its function. tRNA processing of pre-mRNAs snRNA contains genetic code that gets translated into proteins mRNA structural and functional components of the ribosome rRNA contains the anticodon and gets attached to an amino acid

Explanation / Answer

Question 11 - Answer

Part a)

5'-GUAAAUCAUGAGUAGUCAACCUGCCAGC---3'

5'-GUAAAUCAUG, AGU, AGU, CAA, CCU, GCC, AGC---3'

5'-GUAAAUCAUG,   AGU, AGU, CAA, CCU, GCC, AGC---3'

Methionine Serine Serine Glutamine Proline Alanine Serine

Part b)

5'-GUAAAUCAUGAGUAGUUAACCUGCCAGC---3' Mutant mRNA

Part c)

5'-GUAAAUCAUGAGUAGU U AACCUGCCAGC---3'

5'-GUAAAUCAUG, AGU, AGU, UAA, CCU, GCC, AGC---3'

5'-GUAAAUCAUG,   AGU, AGU, UAA, CCU, GCC, AGC---3'

Methionine Serine Serine Stop codon

Part d)

Part e)

Question 12

tRNA - Contains the anticodon and gets attached to an amino acid

snRNA - Processing of pre- mRNAs

mRNA - Contains genetic code that gets translated into proteins

rRNA - Structural and functional components of ribosome

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