MOLECULAR BIOLOGY To answer this question you might want to review how steroid h
ID: 179986 • Letter: M
Question
MOLECULAR BIOLOGY
To answer this question you might want to review how steroid hormones like estrogen function in
cells. Look up the nature, role and location of the estrogen receptor.
You are studying nuclear transport in yeast cells. You identify a conditional mutant which has a defect in nuclear transport at 37°C, but behaves normally at room temperature (22°C). To study this further, you decide to look at transport of a steroid receptor in your mutant cells. You create a plasmid that encodes an Estrogen Receptor Ligand Binding Domain/GFP fusion protein. You transfect the mutant yeast cells within this construct and get the results shown in
What can you conclude from the room temperature data about estrogen receptor transport?
37°C 22°C S? estrogen estrogen Fig. 1. Transport of the Estrogen Receptor in mutant yeast cells grown at room temperature (22°C) or at 37 Cells were treated with or without estrogen hormone, as indicated, and then analyzed by fluorescence microscopy. The white color indicates fluorescence. Grey or black color indicates absence of fluorescence.Explanation / Answer
It can be concluded that in the temperature sensitive mutant, the estrogen receptor fails to localize within the nucleus, when cells are grown at 37 °C but the receptor is trafficked properly to the intra-nuclear compartment when the cells are grown at 22 °C.
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