A. Beginning with intact human pancreatic beta cells, what differences are invol

Question

A. Beginning with intact human pancreatic beta cells, what differences are involved in the construction of a genomic library vs. a cDNA library in bacteria?

b. To achieve stable expressio of pancreatic protein (e.g. insulin) in the bacteria, which library would be more appropriate, and what functional elements would have to be present in the bacterial coloning vector to accomplish this? A. Beginning with intact human pancreatic beta cells, what differences are involved in the construction of a genomic library vs. a cDNA library in bacteria?

b. To achieve stable expressio of pancreatic protein (e.g. insulin) in the bacteria, which library would be more appropriate, and what functional elements would have to be present in the bacterial coloning vector to accomplish this?

b. To achieve stable expressio of pancreatic protein (e.g. insulin) in the bacteria, which library would be more appropriate, and what functional elements would have to be present in the bacterial coloning vector to accomplish this?
b. To achieve stable expressio of pancreatic protein (e.g. insulin) in the bacteria, which library would be more appropriate, and what functional elements would have to be present in the bacterial coloning vector to accomplish this?

Explanation / Answer

A. Genomic library contain fragments of DNA from the entire cell or tissue while cDNA library has only expressed genes sequences.

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