Based on the growth of these amdinocillin resistant clinical isolates grown in M

Question

Based on the growth of these amdinocillin resistant clinical isolates grown in Minimal Media (MM) with and without supplementation, what phenotype is being examined in Table 5? What general term is this phenotype referred to as?

Table 5. Amdinocillin susceptible and resistant clinical isolates, relevant phenotype, growth in various media, and MIC on Mueller-Hinton (MH) plates TABLE 5 Effects of cysteine addition on Amd resistance of clinical isolates" Amd MIC (mgliter) Growth on MM M9 Relevant phenotype No Cys 0.75 mM Cys Strain M9 Cys 0.3 mM Cys DA5438 Cys Yes Yes 0.125 0.125 0.125 DA28439 No 24 0.25 Yes DA14704 Yes No 32 0.125 0.125 DA14709 No Yes 0.38 DA14710 No 0.125 DA14712 No Yes 32 16 0.5 DA14713 No No 0.125 DA14715 No Yes 0.19 16 DA14718 No Yes 32 0.38 DA14719 No Yes 16 0.5 0.094 DA24678 No Yes 48 16 0.38 DA24686 0.19 No Yes 32 16 DA24690 No 32 24 0.125 DA24692 No Yes 0.19 48 Shown are growth on M9 glucose minimal medium (MM) with or without cysteine added and Amd Etest MICson MH plates with different concentrations of cysteine. DA5438 is

Explanation / Answer

The phenotype observed is synthesis of the amino acid Cysteine. The wild type strain which can grow in minimal medium and is able to synthesize the essential amino acid cysteine is called prototroph and the mutant strains which requires cysteine supplementation for growth is called cysteine auxotroph.

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