According to the text above what is the basic principle that allows proteins to

Question

According to the text above what is the basic principle that allows proteins to be separated by electrophersis and than how can this priciple be utilized to detect sickle cell anemia?

Introduction to molecules under the influence in of an is the movement of charged molecules, they migrate electric filed. Because amino acids and proteins are charged form the buffer in an electric field at appropriate pH values. In the most common for the sample is applied to a stabilizing medium that serves as a matrix which the sample The agarose gel is used in the gels electropheretic separation of native (no proteins since low percentage (e.g. 1% agarose) form a sponge-like network which serves as a medium for the buffer, but has pores large enough to allow even the largest proteins to pass unimpeded. is A diagram of the essential components of an agarose system shown in Figure 1. The agarose gel, containing preformed sample wells, is submerged in buffer that is contained within the electrophoretic chamber. Samples to be separated are then loaded into the sample wells. Current from the power supply travels to the negative electrode (cathode), supplying electrons to the conductive buffer solution, gel, and positive electrode (anode), thus completing the circuit.

Explanation / Answer

Answer:

Almost all abnormal hemoglobins can be diagnosed by electrophoresis.

Hemoglobin electrophoresis method exploits the differential charges on different hemoglobins to separate them on the gel. Mutations in hemoglobin amino acids could change the charge on the hemoglobin overall. Therefore such hemoglobins appear distinctly separate in electrophoresis. In sickle cell anemia, there is a change in amino acid composition which affects its mobility on the gel, therefore can be easliy detected.

This test is used as a screening test to identify variant and abnormal hemoglobins.

Examples include HbA1, HbA2, HbF, HbC, HbS etc.

Related Questions

Navigate

• Browse (All)
• Browse A
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.