What biomolecule is source of the fluorescence in your transformed colonies? Giv

Question

What biomolecule is source of the fluorescence in your transformed colonies? Given the source the fluorescence, why don’t the original E. coli colonies or the pGLO plasmid exhibit fluorescence? What biomolecule is source of the fluorescence in your transformed colonies? Given the source the fluorescence, why don’t the original E. coli colonies or the pGLO plasmid exhibit fluorescence? What biomolecule is source of the fluorescence in your transformed colonies? Given the source the fluorescence, why don’t the original E. coli colonies or the pGLO plasmid exhibit fluorescence?

Explanation / Answer

Green fluorescent protein (GFP) is source of the fluorescence in your transformed colonies and the source of the GFP gene is the bioluminescent jellyfish Aequorea victoria.

The orginal E.coli colonies do not have the GFP gene, hence do not exhibit fluorescence. On the other hand, pGLO plasmid which has the gene for GFP needs a host system (eg. E. coli) to express the protein under appropriate conditions because plasmid as such is just an extrachromosomal DNA. Therby, the transformed colonies, E. coli containing the pGLO plasmid under appropriate conditions will exhibit fluorescence.

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