You want see for yourself how mRNA behaves in a eukaryotic cell. You add radioac
ID: 176752 • Letter: Y
Question
You want see for yourself how mRNA behaves in a eukaryotic cell. You add radioactive UTP to the cell medium in two separate dishes. After a short amount of time, you add a large excess of unlabelled UTP to both dishes. You expose Dish A to film immediately after adding the non-labelled UTP, while you wait several hours to expose Dish B. Would the strongest radioactive signal be nuclear or cytoplasmic in dish A? Explain in one sentence. Would the strongest radioactive signal be nuclear or cytoplasmic in dish B? Explain in one sentence. You do a Northern blot using total RNA from dish B and find that one probe results in two strong, distinct bands on the gel. What is the likeliest explanation for this? True or False: The binding of the release factor at the A site of the ribosome when a stop codon is encountered is a signal that leads to the addition of a poly-A tail to the mRNA. Explain your answer.Explanation / Answer
Answer:
Q.a: the strongest signal for radioactive will be in nuclear as the mRNA is synthesised and processed in Nucleus hence the labelled UTP will be more in the nucleus compared to cytoplasm and the thime is very less for the nucleotides to bound.
Q.b. the strongest signal will be in the cytoplasm as the labelled nucleotides will be added to the nucleus and processed in the nucleus and sent to the cytoplasm. hence strong signal will be seen in the cytoplasm.
Q.C: Generally northern blotting is done to findout the expected or desired sequence in the RNA. The appearance of the two strong distinct bands in the gel is due to the presence of the labelled rRNA molecules present in the two subunits of the ribosome. Hence two strong distinct bands were appeared in the gel.
Qd. The above statement made on the mRNA is false.
After the mRNA is translated it will be degraded inside the cell degradation occurs because each mRNA has alife span after this period of time it will be degraded.
After its been detached from the ribosome, it becomes degraded by the nucleases because the cell no longer needs it
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