based on kinetic parameters below for a glucose transporter, glycolytic enzymes,
ID: 176454 • Letter: B
Question
based on kinetic parameters below for a glucose transporter, glycolytic enzymes, and lactate dehydrogenase for patients A and B, what is your major conclusion(s) for each patient?
Vmax(f)
Km glucose
0.01-0.02
0.09-0.10
0.08
0.09
0.02
0.10
Vmax (f)
Km glucose
0.05-0.07
0.10-0.30
0.50
0.18
0.09
0.12
Vmax (f)
Km F6P
Km ATP
0.02-0.05
0.50-0.75
0.45-0.65
0.27
0.40
0.55
0.05
0.55
0.55
Vmax (f)
Km DHAP
0.50-0.80
1.0-3.0
0.58
1.9
0.70
2.5
Vmax (f)
Km GAP
Km NAD+
0.90-1.20
0.20-0.50
0.09-0.20
1.00
0.29
0.10
1.10
0.25
0.09
Vmax (f)
Km PEP
3.0-5.0
0.30-0.60
13.8
0.40
5.0
0.3
Vmax (f)
Km Pyr
8.0-12.0
0.20-0.50
21.8
0.45
13.0
0.20
enzyme kinetic parameter average A B glucose transporter-1 (GLUT-1)Vmax(f)
Km glucose
0.01-0.02
0.09-0.10
0.08
0.09
0.02
0.10
hexokinase (HK)Vmax (f)
Km glucose
0.05-0.07
0.10-0.30
0.50
0.18
0.09
0.12
phosphofructose kinase (PFK)Vmax (f)
Km F6P
Km ATP
0.02-0.05
0.50-0.75
0.45-0.65
0.27
0.40
0.55
0.05
0.55
0.55
triose phosphate isomerare (TPI)Vmax (f)
Km DHAP
0.50-0.80
1.0-3.0
0.58
1.9
0.70
2.5
glyceraldehyde 3-phosphate dehydrogenase (GAPDH)Vmax (f)
Km GAP
Km NAD+
0.90-1.20
0.20-0.50
0.09-0.20
1.00
0.29
0.10
1.10
0.25
0.09
pyruvate kinase (PK)Vmax (f)
Km PEP
3.0-5.0
0.30-0.60
13.8
0.40
5.0
0.3
lactate dehydrogenaseVmax (f)
Km Pyr
8.0-12.0
0.20-0.50
21.8
0.45
13.0
0.20
Explanation / Answer
The Km of the glucose transporter is comparable in both patients. However, consistently rate of reactions are much faster in A relative to B. Though A has a low affinity for glucose as its hexokinase has a higher Km, but it makes u by its much high Vmax. Glycolysis is occurring at a smooth rate in A. the end product pyruvate also gets reduced to lactate under anaerobic conditions in A by the action of lactate dehydrogenase. However, in B the reaction rates are much slower. The body may not able to cope up with the glucose demand especially under heavy physical exercises.
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