21. Which statement is correct? a) Site-directed mutagenesis is considered as on

Question

21. Which statement is correct?

a) Site-directed mutagenesis is considered as one of the reverse genetic approaches because intend to substitute specific amino acid and evaluate the phenotypic effect of the substitution.

b) T-DNA insertion approach is a knock-out mechanism in Arabidopsis that disrupts the gene of interest and the gene can no longer be transcribed into a functional mRNA. As such, this is an approach which works based on post-transcriptional regulation of gene expression.

c) In overexpression approach, we can only increase expression of a gene through moficiation in its transcriptional regulation whereas in gene-silencing approach we can create modification in both transcriptional level and post-transcriptional level.

d) Overexpression is a typical example of post-transcriptional control of overexpressing of a gene

e) CRISPR-Cas approach is a system that works based on the concept of post-transcriptional modification of gene expression which degrades ribosomal RNA.

f) a and c both are correct.

g) a and e both are correct

Explanation / Answer

Option a and c is right

In over expression approach we can increase expression of genes because it is Control manner.

By this we can regulate the post transcription level

And transcription level.

By both regulating this we can increase expression of a gene

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