For each sentence, fill in the missing term/phrase in each blank. Three essentia

Question

For each sentence, fill in the missing term/phrase in each blank. Three essential elements for a plasmid vector are an origin of replication, ______ and ______. In shotgun sequencing, chromosomes are made into small fragments by ______, ______ and then sequenced fragments are aligned by a computer to create ______. In a PCR reaction, the temperature step between 50 and 65 degree C is for ____. A gene that is used in place of the usual coding region to make a visualized protein product is called a _____ in experiments of regulatory DNA.

Explanation / Answer

1. Three essential elements for a plasmid vector are an origin of replication, MCS region( multiple cloning site needed for cloning), and Antibiotic selection marker for selection in presence of proper antibiotic containing plate.

2. In Shotgun sequenceing, chromosomes are made into small fragments by shearing ( or Restriction digestion) and then sequenced fragments are aligned by a computer to create over lapping sequence fragments or segments.

3. In PCR reaction the temparature step between 50 and 60 is for Primer annealing step. the primers attach, or anneal, to the DNA template. PCR requires two different primers, one that can attach to each strand of the DNA molecule.

4. A gene that is used in place of the usual coding region to make a visualized protein product is called a recombinant gene or gene of interest or coding sequence in experiments of regulatory DNA.

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