PCR based techniques car be used to detect viral diseases very soon after infect
ID: 175073 • Letter: P
Question
PCR based techniques car be used to detect viral diseases very soon after infection since it can amplify miniscule amounts of viral based DMA tor detection. For example. it is often used to detect infection of exposed health care workers to HIV soon after the exposure event. The standard HIV test is for antibodies against HIV in Wood and requires on acquired immune response that con take weeks to months Whet technique could be used to doted antibodies against the HIV virus in a sample? PCR Blood cell fractionation and centrifugation Western Blotting Light microscopy of infected cellExplanation / Answer
Ans. Correct option. C. Western blotting.
Western blotting technique is used to detect the presence of the target protein among hundreds of other proteins.
Antibodies against HIV virus are proteins (immunoglobulins), thus can be detected used Western blot.
Option A. Incorrect. PCR amplifies DNA. It can detect proteins.
Option B. Incorrect. Blood cell fraction and its centrifugation may theoretically yield thousands of biomolecules. It is not a technique to detect a protein.
Option D. Incorrect. Antibodies can’t be visualized and differentiated from each other using light microscope.
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