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The polymerase used in PCR is called Taq polymerase and is isolated from the hot

ID: 175067 • Letter: T

Question

The polymerase used in PCR is called Taq polymerase and is isolated from the hot pool bacteria, Thermus aquaticus. Why would human polymerase not be suitable PCR based methods to determine if infection with Ebola had occurred? Choose all that apply. Human polymerase would not recognize viral DNA. It is unethical to harvest human polymerase. Human polymerase is much larger and more complicated as compared to bacterial polymerase. Human polymerase would denature under the conditions of PCR. Human polymerase would get infected by Ebola DNA and not catalyze the reaction.

Explanation / Answer

Polymerise chain reaction require high temperature conditions for DNA replication, therefore Taq polymerase is used which can withstand the temperature, while human polymerase cannot.

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