2. The enzyme pyruvate kinase is another good example of a coupled reaction and
ID: 174859 • Letter: 2
Question
2. The enzyme pyruvate kinase is another good example of a coupled reaction and also occurs in glycolysis. Here, ATP is formed by transfer of a phosphate from PEP to ADP. What is the ?G°' for the formation of ATP from PEP? Circle the correct answer.
PEP + ADP ? pyruvatae + ATP
(a) 0.0 kJ/mol
(b) +31.4 kJ/mol
(c) -31.4 kJ/mol
(d) +92.4 kJ/mol
(e) -92.4 kJ/mol
To answer questions 1-4, consult these values for hydrolysis of phosphorylated compounds phosphoenolpyruvate (PEP) pyruvate P 61.9 kJ/mol -30.5 kJ/mol ATP ADP Pi -13.8 kJ/mol glucose-6-phosphate glucose Pi -43.1 kJ/mol creatine phosphate-creatine Pi -9.7 kJ/mol glycerol 3-phosphate glycerol P 1,3-bisphosphoglycerate 3-phosphoglycerate Pi -49.0 kJ/molExplanation / Answer
Ans c) -31.4 kJ/mol
PEP + ADP ATP + pyruvate Go’ = -61.9 kJ/mol + 30.5 kJ/mol = -31.4 kJ/mol
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