Use the 12bp DNA sequence of the template strand given below to answer the quest
ID: 173727 • Letter: U
Question
Use the 12bp DNA sequence of the template strand given below to answer the questions that follow: 3' - GAT AAA CTT AAG - 5' a) What is the corresponding mRNA sequence? (in the 5' to 3' direction) b) What is the amino acid sequence of the encoded peptide? c) Assume that the 3' base in the DNA sequence of the template strand mutates from G to A. In terms of its effect on the amino acid sequence of the encoded protein, what type of base substitution is this? In terms of the types of nitrogenous bases (purines vs. pyrimidines) involved, what type of substitution is this? d) Assume that a single base insertion occurred at the position indicated at the arrow (between A and T). What type of mutation is this? What would the effect(s) be on the encoded protein?Explanation / Answer
(a) mRNA= 5-'CUA UUU GAA UUC-3'
(b) Leu-Phe-Glu-Phe
(c) G to A is purine to purine substitution (Transition mutation)
It is a Silent mutation since new sequence codes for same amino acid
(d) This insertion would result in shifting of reading frame, causing different peptide to be formed.
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