You\'ve set up a test tube that contains all of the eukaryotic translational mac

Question

You've set up a test tube that contains all of the eukaryotic translational machinery (so, prokaryotic tRNAs, aminoacyl tRNA synthetases, and ribosomes) and some radioactively labeled amino acids. You throw some human Mbd gene mRNA into this tube and you find that you've produced a bunch of radioactive Nbd protein, which is great news for your new experiment. However, for your experiment, you also need some radioactive OMG protein, which is coded by the E. coli OMG gene. If you threw in an mRNA OMG transcript isolated from E. coli cells, would you expect to see radioactive OMG protein? Why or why not? (2-3 sentences)

Explanation / Answer

Nbd protein ie; nuclear (nucleotide) binding domain is the proten that binds to nucleotides. Utilization of amino acids takesplace in the translation process to form the protein. Thus radioactive aminoacids available for translation are used and a radioactive protein is formed. When the OMG gene transcript from E.coli is introduced, it will initiate protein formation ie: translation for OMGP (Oligodendrocyte Myelin Glycoprotein), The translation process will utilized the available aminoacids. As radioactive labelled aminoacids are present, those will be utilized to form a radioactive labelled protein.

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