What is the experimental basis for concluding that puffs in polytene chromosomes

Question

What is the experimental basis for concluding that puffs in polytene chromosomes and loops in lampbrush chromosomes are areas of intense transcription of RNA?

Electron microscopy of RNA-polymerase combined with biochemical analysis have shown a high levels of fluorescence of RNA incorporation, indicating intense transcription.

Radioactively labeled RNA precursors followed by autoradiography have shown a high rate of RNA incorporation, indicating intense transcription.

Fluorescent antibodies against RNA polymerase have shown high levels of fluorescent RNA incorporation, indicating intense transcription.

Density analysis of RNA-polymerase has shown a high rate of RNA incorporation, indicating intense transcription.

Electron microscopy of RNA-polymerase combined with biochemical analysis have shown a high levels of fluorescence of RNA incorporation, indicating intense transcription.

Radioactively labeled RNA precursors followed by autoradiography have shown a high rate of RNA incorporation, indicating intense transcription.

Fluorescent antibodies against RNA polymerase have shown high levels of fluorescent RNA incorporation, indicating intense transcription.

Density analysis of RNA-polymerase has shown a high rate of RNA incorporation, indicating intense transcription.

Explanation / Answer

C. Fluorescent antibodies.

Since these tags specifically binds to the RNAP. Electron micrograph showed only the presence of polytene and lampbrush chromosome.

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