2. (6pts) According to Prosite http://prosite.expasy.org/, alkaline phosphatase

Question

2. (6pts) According to Prosite http://prosite.expasy.org/, alkaline phosphatase (ALP) active site consists of nine amino acids that follow these rules:
(I or V)-any-D-S-(G, A, or S)-(G, A, S, or C)-(G, A, S, or T)-(G or A)-T
a) What is the Prosite pattern for the ALP active site?

b) What is the corresponding Perl regular expression?

3. (6pts) The signature for C2H2-type zinc finger motif is as follows: C-(any 2 to 4)-C-(any 3)-(L, I, V, M, F, Y, W, or C)-(any 8)-H-(any 3 to 5)-H a) What is the Prosite pattern for the zinc finger signature?
b) What is the corresponding Perl regular expression?

Explanation / Answer

2a The prosite pattern for ALP active site is [IV]-x-D-S-[GAS]-[GASC]-[GAST]-[GA]-T.

3a the prosite pattern for zinc finger signature is C-x(2,4)-C-x(3)-[LIVMFYWC]-x(8)-H-x(3,5)-H.

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