The sequence below represents DNA sequence of a small hypothetical protein-codin

Question

The sequence below represents DNA sequence of a small hypothetical protein-coding eukaryotic gene X. Nucleotides in red match to mature mRNA of the wild type copy of gene X. Based on protein sequence analysis you know that ATG in bold is the beginning of the CDS. Underlined areas indicate locations of mutations identified in some individuals. Based on your sequence analysis, answer questions below. Is this the template or non-template strand? Find, circle and name promoter element(s) and polyadenylation signal What is the name of the region in black font between two red segments? How long (# amino acids) is protein encoded by the wild type copy of gene X? Is this the template or non template strand? Find, circle and name promoter element(s) and polyadenylation signal What is the name of the region in black font between two red segments? How long (# amino acids) is protein encoded by the wild type copy of gene X? How long would the encoded protein be in Mutant #4? Predict whether these mutations might impair the function of the gene X. Propose a rationale/mechanism for each prediction

Explanation / Answer

1)Given strand is the non-template strand as it matches to the mRNA sequence

2) The first underlined string is the promoter region.

3)The black segment between the two red (coding) segments is the non-coding intronic region

4)Count the nucleotides in red and divide by 3, will give the number of amino acids encoded by the wild-type protein

5) This mutation would lead to premature stop codon TAG and thus the number of amino acids would be all the red colored letters before the stop code divided by 3.

6) mut1- This region is the promoter region. If its mutated, promoter won't be able to recognize and initiate the transcription process

Mut2 -if TATA box is deleted then transcription factors won't be able to bind at the TATA box and thus RNA polymerase II initiation complex won't be complete.

Mut 3 will lead to change in amino acid

Mut 4 will lead to a premature stop codon TAG

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