in vitro time-kill activity 40 100 10 102 PHA Fig. 6. Quantitative assessment of
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in vitro time-kill activity 40 100 10 102 PHA Fig. 6. Quantitative assessment of membrane permeability: Cells we Table 2 Percentage of fluorochrome-stained cells evaluated by flow cytometry. Sample Stained cell EB 3.5 t 0.0001 Autofluorescence 0,7 0.007x 140 t 0,008 Control G. glabra treated 50.4 1.009% 56.8 t 2.08 Glycyrrhizic acid 63.9 t 2.109 439 t 2.109 Amikacin treated 48.3 t 1.04 48 t 1.009 100 Glycymbizic acid 10 10 100 150 Fig. 7 Mechanism of action of antibiofilm activity. Biofilm treated with standard (Ciprofloxacin) herbal and pure compound for 22 h. Bars represent the average of 3 independent assays ESD. ports the s of the bacteria d thExplanation / Answer
Glycyrrhiza glabra is fouind to inhibit P. aeruginosa at known concentrations. This G.glabra has nitric acid scavenging property more than that of gallic acid normally used as strandard. The G.glabra also shows hydrogen peroxide scavenging activity there by it has anto oxidant activity .It has potent H2O2 activity at 5 to 121micro gram concentrations. In Fig 4 ; as the concentration of G.glabra increases the the inhibition of micro organism also inhibited. in fig 5 Similar to synthetic drug the growth of micro organisms is not seen in 24h incubation period of the drug. When using G.acid the growth has gone to negative values. In the flow cytometer graph the growth of the micro organism is also decreased when the concentration of G.glabra is increased along with synthetic drug. Biofilms are also reduced when treated with G.glabra similar to synthetic drug. From the graphs , you can conclude instead of using synthetic druds you can prefer herbal drugs.
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