1. A DNA strand has the following sequence: 5’-GATCCCGATCCGCATACATTTACC -3’ a) I

Question

1. A DNA strand has the following sequence: 5’-GATCCCGATCCGCATACATTTACC -3’

a) If this strand is being used as a template strand, in which direction would DNA polymerase slide along this strand to make the daughter strand (from left to right or from right to left)?

b) If this strand was used as a template by DNA polymerase, what would be the sequence of the newly made strand? Indicate the 5’ and 3’ ends of the newly made strand.

c) Which DNA polymerase would be responsible for the synthesis of the daughter strand in E. coli?

Explanation / Answer

a) DNA polymerase work from 5' to 3' direction. So the elongation would take place from right to left.

b) 5' GGTAAATGTATGCGGATCGGGATC- 3'

c)DNA polymerase 1

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