You have isolated three different F-minus E. Coli strains, labeled A, B, and C,

Question

You have isolated three different F-minus E. Coli strains, labeled A, B, and C, that are unable to grow on lactose as a sole carbon source (lac-).To determine which mutation is affecting lactose utilization in each of your mutant strains, you decide to mate them with F' donor strains (1,2,3, and 4) that carry differnt versions of the lac operon on the F' plasmid. The table below indicates whether the merodiploid products from each mating are capable of growing on media with lactose as the sole carbon source (+ = growth, - = no growth). For each of your mutant strains (A, B, and C) label which mutation caused the original lac- phenotype (assume that strains A, B, and C have a SINGLE UNIQUE MUTATION in the lac operon).

3. (21 pts) You have isolated three different F.minus E. coli strains, labeled A, B, and c, hat are unable to grow on as a sole carbon source (lac-). determine which mutation is affecting la utilization in each of your mutant strains, you decide to mate them (1, 2, 3, and 4) that carry with donor strains different versions of the lac operon on the F plasmid. The table below indicates whether the merodiploid products from each mating are capable of growing on media with lactose as the sole carbon source growth no growth). For each of your mutant strains (A, B, and C) label which mutation caused the original lac- phenotype (assume that strains A, B, and Ceach have a single unique mutation in the lac operon). C 1 (wild-type) za 2 (lac O) 2 3 (lac o Lacz 4 (lac o, LacY) 4. (16 pts) while performing research on a new bacterial pathogen, you and Noelle discover an alternative form of cas9 that you name cas140. The new based CRISPR system has 2 important differences from the original Cas9-based system: Cas140 recognizes an "NAA PAM sequence, and the target-specific portion of the guide-RNA is only fihn long Than

Explanation / Answer

Mutations that affect the regulation of lacOc anf LacZ and the mutation are located in the same region of the chromosome. F factor carries this entire chromosomal region, LacOc. Point and deletion mutations can express full length as such in the wild type proteins. This mutation changes location of the enzyme from plasma membrane to cytoplasm due to production of truncated proteins

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