You have isolated 10 mutant yeast strains defective in synthesis of leucine (num

Question

You have isolated 10 mutant yeast strains defective in synthesis of leucine (numbered 1- 10). (Mutants 7 and 9 got contaminated and were discarded). These Leu- mutants do not grow on minimal medium (medium lacking leucine). Your classmate has independently isolated 10 Leu-mutants (numbered 11 through 20). You and your classmate combine your resources to determine how many different genes are represented by your 18 surviving mutant strains. You cross each mutant strain and you include crosses to the wild-type strains. Your results are shown in the genetic complementation table below. ("+" represents growth, represents no growth of

Explanation / Answer

COMPLEMENTATION - occur when two strains of an organism with different homozygous recessive mutation that produce same mutant phenotype.

If the mutation fail to complement they must be in the same gene

mutant 2 & 16. These are showing no complementation, they may concides with every group's.

ANSWER 2 :

Group 1 - 2 & 5 & 6

Group 2 - 2 & 4

Group 3 - 2 & 10

Group 4 - 2 & 3 & 8

Group 5 - 1 & 2

ANSWER 3 : All gene can possibly be involved leucine synthesis except gene present on 2 &16.

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