2. The following is a small stretch of nucleotide sequence from a bacterial mRNA

Question

2. The following is a small stretch of nucleotide sequence from a bacterial mRNA: 5' GGGCCCUGAUGUAACAUAGAUAGG-3 A. What is the sequence of the DNA template from which it was transcribed? (indicate polarity). B. Use the genetic code table in Fig 8.11 of your textbook to determine the amino acid sequences encoded by all three reading frames. Use an asterisk to indicate any stop codons you encounter and continue translating beyond them. Nucleotide seq: 5' G G GCC CUG AUGUA A CAUA G AUA G G-3 RF1: RF2 RF3 C. Would the same information be encoded by the RNA sequence that is complementary to the sequence given above? D. Considering that the RNA sequence is a fragment of an mRNA, answer the questions below and BRIEFLY (10 words or less) explain your reasoning.

Explanation / Answer

Answer:

A. The template DNA strand is transcribed to produce the mRNA.

mRNA: 5' GGG CCC UGA UGU AAC AUA GAU AGG 3'

Template: 3' CCC GGG ACT ACA TTG TAT CTA TCC 5'

B. (The reading frame table. Figure 8.11 has not been provided. So, the 3 reading frames which have been used to translate the sequences below are given)

mRNA: 5' GGG CCC UGA UGU AAC AUA GAU AGG 3'

   Translation:

Reading frame 1:

Reading Frame 2:

Reading frame 3:

C. The complemetary sequence to the above given RNA sequence would translate for a different protein since the overall ORF changes.

Complementary sequence: 5' cct atc tat gtt aca tca ggg ccc 3'

D. i. No, since a translated protein starts with the the initiating Met (M). Here, the protein starts with Gly (G).

ii. No, since it contains only two residues before the stop codon.

iii. It can be the end of an mRNA since it contains the last two residues of the stop codon.

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