( Below, they are both the same 16.2 Figures . However, I couldn\'t upload the f
ID: 166891 • Letter: #
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(Below, they are both the same 16.2 Figures. However, I couldn't upload the first photo without it missing some parts, so I posted another with lower quality)
Diamond = Control. Square Symbol = With CTP. Triangle Symbol = With ATP
Figure 16.2: Kinetics of ATCase in the presence of ATP and CTP
2. The kinetics of the ATCase reaction were examined using increasing concentrations of aspartate, in the presence and absence of CTP and ATP as shown in Figure 16.2. a. What information can you obtain by looking at the shapes of the curves in this figure? b. What kinetic parameters) change in the presence of CTP? What parameter s) do not change What is the significance of these observations? c. Answer question 2b for ATP.Explanation / Answer
2(a) In Figure 16.2, in the control set, with the increasing concentration of aspartate, the activity of enzyme shows sigmoidal behavior. In the absence of substrate (aspartate), the ATCase enzyme activity is less. The binding of aspartate to enzyme increases its activity to a certain level and then it becomes steady to give a sigmoidal shape.
2(b) In the presence of CTP, the initial phase of curve increases. CTP acts as allosteric regulator of ATCase enzyme and inhibits the reaction. Thus, in the presence of CTP, the reaction rate is slowed.
2(c) In the presence of ATP, as seen by the curve, the reaction rate is increased at a very low concentration of aspartate. While, at higher concentrations, the effect is reduced and the enzyme activity reaches the steady state.
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