1. Would you rather use pluripotent stem cells or neural stem cells as a source

Question

1. Would you rather use pluripotent stem cells or neural stem cells as a source of transplantable cells for the treatment of neurodegenerative disorders? Explain why you are choosing one over the other.

2. Neural cell types can be specified from ESCs with retinoic acid, conditioned medium, co-cultures or by 'lineage selection'. Which method would you choose if you had to make neurons from ESCs? Explain your answer by contrasting your method of choice to the other methods.

3. A cell population that you are growing in a culture dish expresses Nanog. However, when cells differentiate into endoderm, Nanog is also expressed in early endoderm cells. How do you make sure the cells in your culture dish are pluripotent stem cells, not endodermal cells?

4. Let's say you overexpressed the transcription factor PPARgamma in an osteoblast. What kind of cell will the resulting cell be? Did you create that cell through the process of reprogramming or transdifferentiation?

Please help!!!!!! I would really appreciate it! :)

Explanation / Answer

Ans 1

Pluripotent cells will be preferred over neural stem cells because Induced pluripotent stem cells are a recent advancement in generation of a new class of pluripotent stem cells which are derived from patients' own skin fibroblasts. They can bemade autologous and can provide a sufficient quantity of cells by culturing , making the use of other stem cells unnecessary.

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