PART 4: LUMINESCENT BACTERIA During your summer months, to escape the heat of Da
ID: 165817 • Letter: P
Question
PART 4: LUMINESCENT BACTERIA During your summer months, to escape the heat of Davis, you work with a group of scientists at the North Pole to study the bacterium E luminescent. This is a bacterium that lives in dark icy waters. Your colleagues have found that during certain times of the year, large populations of E lumines- cent glow. While you were away at school, your colleagues identified a signal peptide that when aattanum IL) External signal Molecule Concentration miRNA PROTEIN ONA Gene Circuit Siena Peptide Regulation Components: Signa Transcription Promoter Molecule Sequence/ Factors Open Reading mRNA Active Protein Regulatory Frame glows GB- (peptide) Site introduced to a population of E. Luminescent in the lab, will cause the bacterium to start glowing. Here are the notes they have written for you to bring you up to speed. can then exit the cell The signal peptide is produced by E. Luminescent. The signal peptide and enter a neighboring E. Luminescent. Thus, overtime, an entire population of E. Lumines will come into contact with the signal peptide. Upon contact with the signal peptide, the bacteria produce a protein Glow Bright (GB). The production of GB can be up- regulated by self-production of the signal peptideExplanation / Answer
1.
When small amount of signal peptide will be added it will express the luminiscent protein GB in small quantity means the luminicity will be there but in less quantity. The GB is transacting molecule which increase the fold change in expression of small peptide. But the amount of signal peptide will be less. In the presence of signal peptide the repressor will not bind to the operator that will lead to synthesis of the GB protein which is reponsible for luminiscent.
2.
In the absence of signal peptide the repressor will bind to the operator that will repress the expression of of the GB protein which is reponsible for luminiscent so there will be no luminiscence.
3.
When large amount of signal peptide will be added it will express the luminiscent protein GB in large quantity means the amount of luminicity will be increase relatively. The GB is transacting molecule which increase the fold change in expression of small peptide so the amount of signal peptide will be more. In the presence more signal peptide the repressor will not bind to the operator that will lead to synthesis of the more GB protein which is reponsible for luminiscent.
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