You crossed a petunia plant with red flowers to a petunia plant with white flowe

ID: 16569 • Letter: Y

Question

You crossed a petunia plant with red flowers to a petunia plant with white flowers. The seeds from this cross produced plants that all had red flowers. When the seeds obtained by selfing these plants are grown, the plants produced either red flowers or white flowers in the ratio 3:1.

Based on protein analysis, an enzyme called 'protein A' was isolated from the red flowers. This protein was not present in the white flowers.

Give genotypes with regards to flower color for the plants: parentals, F1, and F2's.

How would you isolate the gene for protein 'A'?

How would you determine the number of copies of this gene in the genome of petunia? Do you think you will find some form of this gene in the white flowered petunia plant?

What experiments would you do to determine if this gene is transcriptionally or translationally regulated at an organ/cell-specific manner?

Can you design an experiment to validate that the gene that you have isolated does encode for a protein that has a function in red pigment synthesis. (Petunia can be transformed).

Explanation / Answer

You crossed a petunia plant with red flowers to a petunia plant with white flowers. the seeds from this cross produced plants that all had red flowers. When the seeds obtained by selfing these plants were grown, the plants produced either red flowers or white flowers in the ratio 3:1. Based on protein analysis, an enzyme called "protein A" was isolated from the red flowers. This protein was not present in the white flowers.??

Red---------dominat allele-----R

White—recessive allele----------r

red genotype---------------RR ,Rr

and white --------------rr

red flowers to a petunia plant with white flowers

red flowers or white flowers in the ratio 3:1.

By doing test cross, the genotype s of parents are known

RR x rr

All offsrings are red

Rr x rr

50% offsrings are red

50% offsrings are white

because of dominace of Red---------dominat allele,tranciption of White(recessive allele r) is blocked during protein synthesis

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You crossed a petunia plant with red flowers to a petunia plant with white flowe

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