Maize gene V is necessary for synthesis of chlorophyll B. A Ds element is insert

Question

Maize gene V is necessary for synthesis of chlorophyll B. A Ds element is inserted inside V. Like other null mutations of V, the Ds-tagged allele (v-Ds) is recessive and results in viable plants with a light green leaf color. A dark green plant is heterozygous for the v-Ds insertion allele and also for the Ac transposon, which is present at a single unlinked locus. Ds excision during development leads to dark green spots. In the progeny resulting from selfing this plant, the following ratios are expected for dark green: light green spotted dark: light green 2:1:1 1:2:1 12:3:1 15:1:0 9:3:4

Explanation / Answer

Gametes from a heterozygous plant

V Ac

V Ac-

VDs Ac

VDs Ac-

V Ac

VVAcAc

Dark green

VV AcAc-

Dark green

VVDs AcAc

Dark green

VVDs AcAc-

Dark green

V Ac-

VVAcAc-

Dark green

VVAc-Ac-

Dark green

VVDs AcAc-

Dark green

VVDs Ac-Ac-

Dark green

VDs Ac

VVDs AcAc

Dark green

VVDs AcAc-

Dark green

VDsVDsAcAc light green with green spots

VDsVDsAcAc- light green with green spots

VDs Ac-

VVDs AcAc-

Dark green

VVDs AcAc-

Dark green

VDsVDsAcAc- light green with green spots

VDsVDsAc-Ac- light green

Gametes from a heterozygous plant

V Ac

V Ac-

VDs Ac

VDs Ac-

V Ac

VVAcAc

Dark green

VV AcAc-

Dark green

VVDs AcAc

Dark green

VVDs AcAc-

Dark green

V Ac-

VVAcAc-

Dark green

VVAc-Ac-

Dark green

VVDs AcAc-

Dark green

VVDs Ac-Ac-

Dark green

VDs Ac

VVDs AcAc

Dark green

VVDs AcAc-

Dark green

VDsVDsAcAc light green with green spots

VDsVDsAcAc- light green with green spots

VDs Ac-

VVDs AcAc-

Dark green

VVDs AcAc-

Dark green

VDsVDsAcAc- light green with green spots

VDsVDsAc-Ac- light green

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