You would like to test the adhesion of osteoblasts on different extracellular ma

Question

You would like to test the adhesion of osteoblasts on different extracellular matrix (ECM) proteins. You first spot the ECM proteins directly on glass slides. Then, the osteoblasts are cultured in media containing serum for 2 days on the slides. When you examine the slides, you find that the cells have attached to the entire glass slide (not just your spotted ECM proteins). Explain this result. A coworker gives you another slide with the ECM proteins already spotted. They mention that they first coated the glass slide with Polymer A-silane. What is a surface modification technique that may have been used to alter the slide with the Polymer A-silane? When you culture the osteoblasts under the same conditions, you find that after 2 days the cells now only adhere to the regions of the spotted ECM proteins. Explain why Polymer A prevented the cells from adhering to the glass. How are the cells interacting with the protein-spotted materials? Due to favorable cell-material interactions with spots, what could then occur to the osteoblasts (e.g., what cell function(s) are affected) (there are many answers - only one potential outcome is necessary)? How does the extracellular interaction get to affect cell function?

Explanation / Answer

1. The attraction between a negatively charged slide and a negatively charged osteoblast is mediated by charged proteins with a distinctive quadrupolar internal charge distribution. Osteoblasts undergo differentiation to form bone stromal cells. ECM protein mediated enhancement of proliferation, osteoblast differentiation.

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