As a researcher investigating structure-function questions about DNA polymerase,

Question

As a researcher investigating structure-function questions about DNA polymerase, you are interested in isolating E. coli mutants with altered DNA polymerase function and then identifying the amino acids altered in the mutants. Based on our current understanding of DNA polymerase function, predict what kind of amino acid/gross structural changes in DNA polymerase will cause the following mutant phenotypes you observe in your cells:
The mutant DNA polymerase:
1)is able to incorporate rNTPs and dNTPs into new strand
2)looses all processivity – shows only one reaction per binding
3)has trouble “catching” new dNTPs – they touch down but often detach again
4)is much more error prone than normal DNA polymerase
5)can’t form a new phosphodiester bond

Explanation / Answer

ANS- 4

main function of DNA polymerase is to involved in the insertion of the nicleotide with the correct sequence and proofreading, the removal ofincorrectly incorporated nucleotides.DNA polymerases allow error prone
DNA synthesis through sites of DNA damage where other polymerases would be unable to function

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