The system for transport of mannitol across the Escherichia coli membrane involv

Question

The system for transport of mannitol across the Escherichia coli membrane involves a membrane-bound enzyme that couples phosphorylation of the sugar to its translocation through the membrane, Fluorescence life-time and anisotropy measurements have been carried out of the tryptophans in this enzyme dissolved in detergent micelles [D. Dijkstra, J. Broos, A. J. W. G. Visser, A. van Hoek, and G.T. Robillard, Biochemistry 36, 4860 (1997)]. The fluorescence anisotropy decay of one of these tryptophans has two components. The faster component, with a rotational correlation time of about 1 ns, is due to local motion of the tryptophan. The decay of anisotropy for the slower component can be approximated by the following data. Calculate the rotational correlation time. The expected correlation time for the known molecular weight of the macromolecule is 120-140ns. How do you explain the difference between the calculated and observed correlation time? When the protein is phosphorylated, the rotational correlation time increases to 51 ns. What might be the cause of this change?

Explanation / Answer

The correlation is one of the most common and most useful statistics. A correlation is a single number that describes the degree of relationship between two variables.Obeserved is the practical thing done but the calcualted is the method using by the correlation coefficient.The change might be due to correlation coefficient.

Related Questions

Navigate

• Browse (All)
• Browse T
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.