Table 1. Characteristics of cholera Result Can grow at a pH of 10 Experiment or
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Table 1. Characteristics of cholera Result Can grow at a pH of 10 Experiment or observation Can grow 30-40 cannot grow at 4 C Reddish layer on top Optimal Growth Temperature Survives and produces CO2 gas ink n in anaerobic growth chamber Bubbles form rapidly Gram Stain Test strip turns dark ested with Hydrogen Peroxide dase Test 1% minimum up to 6% VGrown with Naci (9% in wt/v) B acid, DL-lactic acid. Carbon Source variety including malate, propionic and D-alanine, L-aspartic acid This not an acado Yout in a high pH a Defined Minimal Medium: 50 mM Tris 50 mM MgSO4 able ma is a mesophile but, 10 mM CaCl2 2. This 300 mM NaCl 10 mM KCI 0.333 mM K2PO4 Indole mcain 18.5 mM Glycerol Positive 32.6 mM NH4CI la Aase The glycerol can be replaced by a fina and can use concentration of 10 mM N-acetyl- a Carlo m en en ay Sovre to glucosamine. Then, do not add ramE) result this is because ammonium chloride merc ink, then has a f gram Stain exha layer preventin kin use O2 as oltrg own, lycerol ars glycogen orExplanation / Answer
Vibrio cholerae is the causative organism of Cholera disease which is one of the most important paper with diseases. Tevar among the common bacteria on surface waters water is the Reservoir for vibrio it causes notifiable epidemic diseases.
Robert koch first observed this pathogen in fecal matter of patients and he named it as vibrio comma in 1984 it was named as vibrio cholerae.
The cells of this organism are typically I would and comma shaped hence the name vibrio cholerae it measures 1.5-3 microns in length and 0.5 microns in with it is actively motile with single long flagellum.
The cells are Gram Negative non capsulated non performing aerobic or facultative anaerobic motile rigid curvedrt rods.
This pathogen and can be easily cultivated on ordinary bacterial media like blood agar macconkey agar. The optimum temperature is 37 degree centigrade optimum pH is 8.2
Most important biochemical characteristics in production the virulence factor is toxigenicity it releases and exotoxin which is having ab model.
It also produces hemolysins.
O and H antigen for present.H antigen is common in all vidreos but 0 antigens are highly specific based on these hundred sero types of identified.
Vibrio cholerae is water borne pathogen a person has to consume 10 power 8 to 10 power 10 cells before getting infected. It enters small intestine attaches to epithelium of regional adhesion is very important and it produces the toxin all the symptoms of due to this production of tops in but not due to growth of pathogen the toxic changes the permeability of the membrane incubation period is 1-3 days the symptoms mainly observed are due to dehydration disease starts with my life and fever skin becomes dry wrinkled muscular cramps especially in arms and legs eyes become Grey the blood in urine production stops blood flow to brain death becomes black.
Diagnosis: specimens are usually stools especially mucus flakes in stools they are used for isolation of pathogen Under The phase contrast oridathu field microscope vibrating moments of the pathogen observed for the confirmation of its identity agglutination test is done.
Therapy: first aid fir cholera is replacement of water and electrolytes by giving oral rehydration solution.
When the patient is recovering II therapy antimicrobial therapy is used tetracyclines are commonly used.
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