Read beginning through the end of section in the article titled: \"Footprints ar

Question

Read beginning through the end of section in the article titled: "Footprints are quantitative markers of factor occupancy" Answer questions in your own words.

Article URL: http://www.nature.com/nature/journal/v489/n7414/full/nature11212.html

Question:

Question contd:

7. (5 points) NRFI I specific regulatory transcription factor. Briefly define NRF1 (use outside resources, not in this paper). Then, in looking at Figure lc, we find "heat maps" that show some relative relationships. There were 4,262 instances of the sequence "motif where NRF1 is known to bind DNA across the genome. These 80 bp ites were compared with DNase cut sites

Explanation / Answer

NRF1 is a transcription factor that acts on nuclear genes encoding respiratory subunits and components of the mitochondrial transcription and replication machinery NRF1 transcription factor binds to 2 palindromic sites within the promoter of the eIF-2-alpha gene and is essential for eIF-2-alpha transcription. It is also involved in heme biosynthesis through regulation of globin gene expression. Majority of NRF 1 motif DNase 1 footprints coincided with the NRF 1 occupancy identified by chIP sequence.This indicates that there is a strong correlation betweenfootprints and chIP sequence Positive correlations exist between footprint occupancy and nucleotide-level evolutionary conservation patterns for NRF1 motif. This indicates that there is a strong evolutionary selection on transcription factor occupancy.

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