Please help urgently and also draw the gels please clerarly to see the answer. A
ID: 163865 • Letter: P
Question
Please help urgently and also draw the gels please clerarly to see the answer. And explain the answer.
1.You are evaluating a gene of interest using Northern (RNA) and Western (protein) blotting. You wish to evaluate the impact of specific miRNAs and siRNAs on the regulation of your gene. You have a specific miRNA and a specific siRNA that should target your gene. You treat different samples of cells with each RNA, and then evaluate the material from these treated cells on blots, in comparison to your control treated cells. Assuming each RNA does regulate your gene, indicate the expected result on the gels, following treatment.
x C chega sudy Guided sol. x Practice problems 8.pdf ama expectea to ana We e ena you eCItatio ate Doing problems will help you learn the material, and do well on the exams. Beyond your specific problem assignment, it is a good idea to do a of the problems below for practice. If you finish these, you can also work the problems in the back of each chapter 1. You are evaluating a gene of interest using Northern (RNA) and Western (protein) blotting. You wish to evaluate the impact of specific miRNAs and siRNAs on the regulation of your gene You have a specific miRNA and a specific siRNA that should target your gene. You treat different samples of cells with each RNA, and then evaluate the material from these treated cells on blots, in comparison to your control treated cells. Assuming each RNA does regulate your gene, indicate the expected result on the gels, following treatment. Northern blot Westem blot 2. The following diagram illustrates a step in the process of translation. Add the HOSEAExplanation / Answer
miRNA and siRNA both binds to mRNA in different ways and leads to its degradation. As a result of it, mRNA is not able to translate itself into protein.
When the cells were treated with siRNA and miRNA, leads to degradation mRNA.
Northern blot detects the presence of mRNA when radiolabelled P32 primers (specific for the gene of interest) were hybridized with the total RNA of the cell. Given miRNA and siRNA were specific against the gene of interest. Upon treatment with miRNA and siRNA, mRNA degraded and there was no band in the northern blot whereas in the case of the control sample cells were treated with either with scrambled miRNA of no miRNA or siRNA due to which one band was visible in the northern blot.
Western blot is a technique used to detect the expression of proteins inside the cells. In western blot, specific antibodies are used which binds only to the protein against which they are developed.
In the given condition when cells were treated with miRNA or siRNA (which are specific for the gene of interest), leads to degradation of mRNA and no translation of the protein of interest. As a result of it when the isolated protein was subjected to western blotting there was no band of the gene of interest. In case of control, sample cells were treated with either with scrambled miRNA of no miRNA or siRNA due to which one band was visible in the western blot
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.