You decide to give the Baroness and her Raests acids. As you go through the card

Question

You decide to give the Baroness and her Raests acids. As you go through the cards Printed with all ofthe codons and their corresponding amino you the and Person will bring their codon and to the front of de room and show think! You begin r sequence of amino acids. This wil be treat. of the start the sequence at site 1557. which specific indicated as the first each corresponds to a codon in the CI. As you are is the position that GenBank and discussing how must have made a mRNA and an amino acid in the protein, you read ahead and notice that your student mistake! underlining the sets of three nucleotides You think, "There is a mistake in the DNA my audience notice? What a sequence Darn it is this going to clhange the protein product Win mess. You need to determine if belt made a mistake. One do this is to use a BLAST search to compare the student's sequence the per quick way to to nih govrBl Use the Specialized to the sequence that is actually in the database. Go sequence above BLAST option: Align two (or more) seq using BLAST (bl2seq. Copy the DNA numbers or (student's sequence) and paste it quences Query Sequence (dont worry about the for about up" the into the box designated as the this for you), Find the correct sequence ying sequence by spaces, BLAST will do the PmoCAB operon from py this sequence and paste it into tell 4456718. the Sequence box on the bl2seq Run comparison (click the BLAST button) and note any subject form. the BLAST differences non-alignment. Now you are ready to go to the next set of questions. Questions 1. dentification of the mistake. If the nucleotides in the gene were changed as indicated in the mistake sequence, what type of would be? Explain. 2. The DNA is sense Usmand double stranded DNA that makes sp the pmoC gene. (Give sequence shown the ofthe the template (-strand DNA is transcribed? the mRNA be when encoding What would when the mRNA is translated? (Give first 15 amino first 60 bases). What would the amino acid sequence be 3. Analysis of the data. How is the protein product of this DNA sequence different from the PmoCthat would be made if the gene did not have a mutation? Make a prediction as to the functionality of this altered protein to

Explanation / Answer

1)The copying of DNA takes place andto be pasted which means Duplication mutation will take place and one or more copies of the DNA willbe abnormally used in this case.The different copies wil be used as it has been mentioned in the problem here.

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