Question Set 7: based on this data below BXC genotype: His-/His- Ura+/Ura- Leu-/

Question

Question Set 7: based on this data below

BXC genotype: His-/His- Ura+/Ura- Leu-/Ura+ MATa/MATalpha

MATa Tester cell geotype: HIS + URA + ARG- MATa( remeber if yeast grows on MAta tester cells they are MAT alpha )

MATalpha Tester cells genotype: His+ Ura+Leu+Arg-MAtalpha

1. If you have a colony of diploid cells from your YPD colony plate, what will be their histidine genotype? (Recall that any diploid cells on your YPD colony plate originally would have come from a BxC mating.) Can these diploid cells grow on SD? Can these diploid cells mate with either of the mating tester strains?

2. Complete the table below..

Hypothetical haploid colony on YPD plate (colony arose from the sporulation of your BxC diploid

MATa mating tester strain

MATalpha tester strain

Histidine Genotype

Arginine Genotype

Ability of cell to grow on SD

3. Suppose you have a patch of MATalpha haploid cells on your YPD patch plate.   If these cells mate with the cells from the MATa tester cells, what will be the histidine and arginine genotypes of the resulting diploid? Will this new diploid grow on SD.

4. Suppose you have a patch of MATa haploid cells on your YPD patch plate.   If these cells mate with the cells from the MATalpha tester cells, what will be the histidine and arginine genotypes of the resulting diploid? Will this new diploid grow on SD.

5. All of your YPD patches will be replica printed onto a MATa lawn and onto a MATalpha lawn. Each of those replicas will be incubated for 48 hours and then printed onto SD. You will therefore have two SD plates, one originating from the MATa tester replica and one originating from the MATalpha tester replica.  

a. Should any of the specific patches that arise on the SD plate originating from the MATa tester replica also arise on the SD plate originating from the MATalpha tester replica?

b. If you have a diploid patch on your YPD patch plate, will that colony result in growth on the SD plate originating from the MATa tester replica

c. If you have a diploid patch on your YPD patch plate, will that colony result in growth on the SD plate originating from the MATalpha tester replica?

6. Suppose you observe a patch growing on the SD plate originating from the MATa tester replica. What was the mating type of the original colony on the YPD colony plate?

7. Suppose you observe a colony growing on the SD plate originating from the MATalpha tester replica. What was the mating type of the original colony on the YPD colony plate?

Hypothetical haploid colony on YPD plate (colony arose from the sporulation of your BxC diploid

MATa mating tester strain

MATalpha tester strain

Histidine Genotype

Arginine Genotype

Ability of cell to grow on SD

Explanation / Answer

1..The colony of yeast cells which is produced from original YPD plate will have genotype--His-/His-,Ura+/Ura-,leu-/Ura+(MATa/MAT alpha).Moreover these cells do not grow of synthetic defined media(SD) .Furthermore the histidine and uracil metabolism present here will not take place as the strain present over here is mutant.

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