FGF2 can bind to both FGFR1c and to heparan sulfate proteoglycans (HSPGs) simult

Question

FGF2 can bind to both FGFR1c and to heparan sulfate proteoglycans (HSPGs) simultaneously. The bi nding of FGF2 to FGFR1c has a K D = 10 nM, a nd the binding of FGF2 to HSPGs has a K D = 2 00 nM. The forward binding rate constant (k f ) for binding of FGF2 to either FGFR1c or HSPG is the same and is equal to 1 x 10 6 M - 1 min - 1 whereas the k f for binding of FGF2 to both FGFR1c and HSPG simultaneously is equal to 2 x 10 6 M - 1 min - 1 . Based on this information what might the K D for the simultaneous binding of FGF2 to FGFR1c and HSPG be? Explain your answer

Explanation / Answer

FGF2 can bind to both FGFR1c and to haparan sulfate proteoglycans simultaneously.

Given:-

The binding of FGF2 to FGFR1c has a kD= 10nM

The binding of FGF2 to HSPGs has a kD= 200 nM

That indicates the binding capacity of FGF2 to FGFR1c is 20 times more than the HSPGs. Also given-:

Forward binding rate constant (kf) of FGF2 to either FGFRc1 or HSPG is same and is equal to 1x 106 M-1 min-1.

The kf for binding of FGF2 to both FGFRc1 and HSPG simultaneously is equal to 2x106 M-1 min -1 .

So it indicates the kf for binding of FGF2 to both FGFRc1 and HSPG simultaneously is (1x10-6 + 1x10-6 ) =2x10-6 M-1 min-1

Therefore the kD for the simultaneous binding of FGF2 to FGFRc1 and HSPG is 10nM + 200n M

=210nM that is sum of both the forward binding constant

Kf= 210nM

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