Given the wobble rules, what is the fewest number of tRNAs necessary to insert t

Question

Given the wobble rules, what is the fewest number of tRNAs necessary to insert the amino acid Leu into a peptide, and what is the anticodon for each tRNA? There are six codons that specify Leu: UUA, UUG, CUU, CUC, CUA, CUG. Keep in mind all sequences in this question are written 5' to 3'

A) two, with anticodons IAA and GAG

B) two, with anticodons UAA and IAG

C)three, with anticodons IAA, GAG, and UAG

D) three, with anticodons UAA, GAG, and UAG

E)three, with anticodons UAA, IAG, and UAG

A researcher isolates a mutant variant of the bacterial translation factor EF-G. The mutation allows proper folding of the protein and binding of GTP, but does not allow GTP hydrolysis. At what stage would translation be blocked by this mutant protein?

A)Binding of fMet-tRNAfMet to P site.

B) Second aminoacyl-tRNA binding to the ribosomal A site.

C) Formation of peptide bond between amino acids.

D) Movement of ribosome along the mRNA to allow binding of a new EF-Tu–tRNA in A site.

Given the wobble rules, what is the fewest number of tRNAs necessary to insert the amino acid Leu into a peptide, and what is the anticodon for each tRNA? There are six codons that specify Leu: UUA, UUG, CUU, CUC, CUA, CUG. Keep in mind all sequences in this question are written 5' to 3'

A) two, with anticodons IAA and GAG

B) two, with anticodons UAA and IAG

C)three, with anticodons IAA, GAG, and UAG

D) three, with anticodons UAA, GAG, and UAG

E)three, with anticodons UAA, IAG, and UAG

A researcher isolates a mutant variant of the bacterial translation factor EF-G. The mutation allows proper folding of the protein and binding of GTP, but does not allow GTP hydrolysis. At what stage would translation be blocked by this mutant protein?

A)Binding of fMet-tRNAfMet to P site.

B) Second aminoacyl-tRNA binding to the ribosomal A site.

C) Formation of peptide bond between amino acids.

D) Movement of ribosome along the mRNA to allow binding of a new EF-Tu–tRNA in A site.

Explanation / Answer

1.D) three, with anticodons UAA, GAG, and UAG

There are six leucine codons:UUA,UUG,CUU,CUC,CUA,and CUG.The anticodon AAU would recognize UUA and UUG.You would need two other tRNAs to efficiently recognize the other four leucine codons.These could be GAG and GAU or GAA and GAU.

2.b)Second aminoacyl-tRNA binding to the ribosomal A site.

IF-2:the 70S ribosome would form,but initiation factors would not be released and elongation could not Start.
EF-Tu:the second aminoacyl-tRNA would bind to the ribosomal A site,but no peptide bond would form.
EF-G:the first peptide bond would form,but the ribosome would not move along the mRNA to vacate the A site for binding of a new EF-Tu-tRNA.

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