FGF2 can bind to both FGFR1c and to heparan sulfate proteoglycans (HSPGs) simult

Question

FGF2 can bind to both FGFR1c and to heparan sulfate proteoglycans (HSPGs) simultaneously. The bi nding of FGF2 to FGFR1c has a K D = 10 nM, a nd the binding of FGF2 to HSPGs has a K D = 2 00 nM. The forward binding rate constant (k f ) for binding of FGF2 to either FGFR1c or HSPG is the same and is equal to 1 x 10 6 M - 1 min - 1 whereas the k f for binding of FGF2 to both FGFR1c and HSPG simultaneously is equal to 2 x 10 6 M - 1 min - 1 . Based on this information what might the K D for the simultaneous binding of FGF2 to FGFR1c and HSPG be? Explain your answer.

Explanation / Answer

FGF2 can bind to both FGFR1C and to heparan sulfate proteoglycons(HSPGs) simultaneously.

the binding of FGF2 to FGFR1c has a KD=10nM

the binding of FGF2 to HSPGs has a KD=200nM

the forward binding rate constant(kf) for binding of FGF2 to either FGFR1c or HSPG is same and equals to 1 x10^6M-1 min-1

whereas the kf for binding of FGF2 to both FGFR1c and HSPG simultaneously is equal to 2 x10^6M-1min-1

so the KD for the simultaneous binding of FGF2 to FGFR1c is 20 nM

and  for the simultaneous binding of FGF2 to HSPGs is 400nM

why because simultaneous Kf for binding values are doubled to the forward binding rate constant(kf) for binding values.

Related Questions

Navigate

• Browse (All)
• Browse F
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.