1. Which physical effect limits the resolution of an optical microscope (A) Refl

Question

1. Which physical effect limits the resolution of an optical microscope (A) Reflection (B) Diffraction (C) Interference (D) Scattering 2. Which of the following is true about the spherical aberration (A) A spherical aberration occurs when a white light is used. (B) Sample refractive index mismatch is the major cause of spherical aberration. (C) A spherical aberration occurs when different focusing occurs at two perpendicular plane (D) A spherical aberration comes from the off-axis light propagation. 3. Numerical aperture of an objective lens describes the (A) ability of an optical system to show two close objects as separate (B) ability of an optical system to magnify an image (C) ability of an optical system to estimate the size of an image (D) size of an image 4. Which of the following component is NOT the properties of light: (A) Only the intensity of the light can only be detected. (B) Phase shift cannot be measured. (C) Light belongs to electromagnetic wave (D) Light is detected as particles 5. Dark field microscopy is (A) A scanning microscopy technique (B) A technique based on contrast agents. (C) Equivalent to spatial high-pass filtering at the Fourier plane (D) Equivalent to spatial low-pass filtering at the image plane

Explanation / Answer

1. Diffraction.

2. ->B , sample refractive index mismatch is the major cause of spherical abberation.

3. ability of an optical system to estimate the size of an object.

4. Phase shift cannot be measured, is wrong.

5. A technique based on contrast agents.

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