You have a solution with a protein concentration of 12 mg mL -1 . The total volu
ID: 152180 • Letter: Y
Question
You have a solution with a protein concentration of 12 mg mL-1. The total volume of this solution is 18 mL. What is the total amount of protein (in mg to 2 s.f.)?
The molecular weight of ammonium sulfate is 132.14 g mol-1 and you have a solution of total volume 9 mL. What weight of ammonium sulfate should you add to reach a final concentration of 1.5 M (in grams to 2 s.f.)?
What is the pH of the buffer you will use in the anion-exchange chromatography purification step in your purification of pyruvate kinase?
Explanation / Answer
You have a solution with a protein concentration of 12 mg mL-1. The total volume of this solution is 18 mL. What is the total amount of protein (in mg to 2 s.f.)?
Concentration= mass/volume
mass of protein= concentration * volume
= 12*18
= 216 mg
Correcting to 2 significant figures ~ 220 mg
The molecular weight of ammonium sulfate is 132.14 g mol-1 and you have a solution of total volume 9 mL. What weight of ammonium sulfate should you add to reach a final concentration of 1.5 M (in grams to 2 s.f.)?
We know Molarity(M)= mass/(molecular weight*volume)
therefore, required mass to be addes
= Molarity* molecular weight* volume in litre
=1.5*132.14*(9/1000)
as 9 ml = (9/1000) l
= 1.8 g
What is the pH of the buffer you will use in the anion-exchange chromatography purification step in your purification of pyruvate kinase?
For pyruvate kinsae, buffer pH should be about 7.5 as it is necessary to its required pH of the body's metabolic reactions.
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