You want to analyze DNA samples on an agarose gel. Before loading the sample int

Question

You want to analyze DNA samples on an agarose gel. Before loading the sample into the gel, you need to add loading buffer. The concentration of the stock loading buffer (LB) is 6X. How much loading buffer do you need to add to your sample to give a final concentration of 1X for a. 20 ul restriction enzyme digest b. 10 ul PCR product
Please show all work and units thank you! You want to analyze DNA samples on an agarose gel. Before loading the sample into the gel, you need to add loading buffer. The concentration of the stock loading buffer (LB) is 6X. How much loading buffer do you need to add to your sample to give a final concentration of 1X for a. 20 ul restriction enzyme digest b. 10 ul PCR product
Please show all work and units thank you! a. 20 ul restriction enzyme digest b. 10 ul PCR product
Please show all work and units thank you!

Explanation / Answer

Since its 6x (6 times concentrated), it should be diluted to a 1:6 ratio, so 1ul of loading buffer is added for every 5ul of reaction to give a total volume of 6ul(1ul buffer + 5ul reaction product). Now the buffer is diluted to 1X concentration (1ul buffer in total 6ul volume).

a. for 20 ul restriction enzyme digest - loading buffer added = 20/5 = 4ul

b. for 10 ul PCR product - loading buffer added = 10/5 = 2ul

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