Use the following DNA Sequence to answer the question in this assignment DNA: T

Question

Use the following DNA Sequence to answer the question in this assignment

DNA:     T A C C A C G C C A A G C G A A G C G G G A T T

mRNA (part 1) -

amino acids (part 3) -

Part 1: Transcription

Transcribe the sequence into mRNA – add complementary bases to the DNA above. Remember there is no T in RNA so use U as a compliment to A.

Separate the mRNA into codons – divide the mRNA into sections of three letters(bases) each

Read the genetic code chart to find the Start and STOP codons. Identify the Start and Stop codons in the mRNA sequence above. Highlight or circle the start and stop codons above.

Name the enzyme responsible for transcription: ____________________________________

Explanation / Answer

PROVIDED DNA SEQUENCE  TAC CAC GCC AAG CGA AGC GGG ATT

PART 1) TRANSCRIPTION   UAC CAC GCC AAG CGA AGC GGG AUU

COMPLIMENTARY BASE AUG GUG CGG UUC GCU UCG CCC UAA

during transcription the mRNA is synthesized from the template DNA under the influence of enzyme known as RNA POLYMERASE.  Using the 3 letter genetic codon table the sequence could be decoded into respective amino acids upon translation which looks like below

AUG (M- METHIONINE) (AUG IS ALSO THE START CODON)

GUG (V- VALINE)

CGG (R- ARGININE)

UUC (F- PHENYLALANINE)

GCU (A- ALANINE)

UCG (S- SERINE )

CCC (P- PROLINE)

UAA (-) (UAA IS ALSO THE STOP CODON)

Using the genetic code chart for the above nucleiotide sequence we could find that START CODON IS (AUG) and the stop codon is (UAA)

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