13b. What aspect of its structure causes a RNA molecule to migrate toward to the

Question

13b. What aspect of its structure causes a RNA molecule to migrate toward to the positive pole? [2 pts) 13c. Based on the gel, was the protein polymerase (17 RNA pol) or the ribozyme polymerase (R18 RNA pol) more active in ice (-7-C)? Explain your interpretation. [2 pts 13d. Imagine you introduced a mutation to the ribozyme polymerase. The mutant is now 17°C but very efficient at extending RNA at-7°C In the gel below, draw the banding pattern you will inactive at expect from the RNA synthesis assay using the mutant ribozyme. [6 pts] T (oC) 17 17 -7 -7 t (days) 1 8 18 R18 ribozyme RNA pol mutant

Explanation / Answer

Answer:

a.In gel electrophoresis method, the mixtureof DNA, RNA, and proteins are to be seperated. Here, gel electric field is applied such that one end of the gel has a positively charge and the other end has a negative charge. Because both DNA and RNA are negatively charged molecules,they will be pulled toward the positively charged end of the gel.

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