Figure 4-16 External view (a) and diagram (b) ot a scning MATERIALS Per student

Question

Figure 4-16 External view (a) and diagram (b) ot a scning MATERIALS Per student group: - photographs of TEM micrographs (negatives) or digital images - photographs of SEM negatives or digital images PROCEDURE 1. Examine some photographs of TEM micrographs (negatives) or digital images. The darker areas are m electron-dense in the specimen than the lighter areas Now look at some photographs of SEM negatives or digital images. The lighter areas correspond to emission of greater numbers of secondary electrons from that part of the specimen; the darker areas e 2. less. 3. What type of microscope (compound light, dissecting, phase-contrast, TEM, or SEM) would you us examine the specimens listed in Table 4-7? TABLE 4-7 Microscope Use Specimen Living surtace of the finger Dye-stained slide of a section of the finger Gold-coated bacteria on a single cell of the finger Unstained section of a biopsy from the finger Heavy metal-stained, very thin section of the finger Microscope ngour eomeund Micro

Explanation / Answer

electron microscope for living surface of finger compound light microscopy for dye stained slide of a section of a finger scanning electron microscopy for gold coated bacteria compound light microscopy for unstained dection of a biopsy from a tissue. Transmission electron microscopy heavy metal stained thin section of a finger.

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