Pre-Lab Assignment #4-Simple, Negative, and Gram Staining Late Submissions are u
ID: 150490 • Letter: P
Question
Pre-Lab Assignment #4-Simple, Negative, and Gram Staining Late Submissions are unacceptable. NAME: Match the definition to the word it correctly describes. Answers can be used more than once. A. This procedure will kill and adhere the specimen 1. 1. Chromophore 2. Basic stains 3. Auxochrome 4. Heat-fixing 5. Chromogen 6. Acidic stains to the slide, and make cellular proteins easier to see; however, it may shrink the cell and distort the organism's cellular shape and arrangement B. This type of stain has a negatively charged auxochrome because it lost a H C. This is the charged portion of the chromogen. D. This type of stain usually bonds with H' or loses a OH and becomes positively charged. E. This is the portion of the chromogen that has color. F. This type of stain is attracted to the negative charge of the bacterium and will dye the bacterium a certain color and leave the background white. G. This is the colored molecule of a stain that (usually is a benzene derivative) H. This type of stain repels the negative charge of the bacterial cell, thus leaving the organism unstained with a colored background Identify the following stains as being basic (B) or acidic (A): a. Eosin b. Safranin_ c. Methylene blue d. Nigrosin e. Crystal violet f. India ink TRUE FALSE Acidic stains can also be called negative stains.Explanation / Answer
Answer-(1)
A)Heat fixation, this process make specimen fixed to glass slide ,kill organism and overfixation leads to distortion of structure.
B) Acidic strain- Acids are those substances that lost hydronium ion and become negatively charged.
C)Chromophore, it is the charged portion that give colour.
E) chromophore impart colour.
F) Basic dye bind with positive charge on bacteria.
G)chromophore, benzene derived.
H) eosin, negatively charged so repel.
Answer 2
A)Acidic
B) basic
C) basic
D) acidic
E)basic
F) acidic
Answer 3 - True, acidic stain are negative because it give hydrogen ion and become negatively charged.
Answer 4
Reasons to stain microorganisms
A) To differentiate between gram positive and gram negative
B) to identify organism.
C) to identify bacilli from cocci
D) for treatment purpose.
Answer 8) crystal voilet is primary stain in grams staining.
Answer 9) safrannin is counter stain in grams staining.
Answer10) Acetone is decolouriser in grams staining.
Answer11) Time required for each stain is 1-2 minute.
Answer12) gram positives are voilet in colour.
Answer 13) gram negatives are purple or red in colour.
Answer 14)Iodine act as mordant, it form dye iodo complex .
Answer15) No because tap water may give false result, may cause contamination ,
Answer 16)less peptidoglycan and more lipid
Answer17)No gram negatives and gram positives have different susceptibility to different antibiotics.
Answer 20) differnt differential stains are -
A) Acid fast stain
B) Albert stain
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