The above screen shot is already done Part A : HAEIII Part B: EcoRI & HINDIII Th

Question

The above screen shot is already done

Part A : HAEIII

Part B: EcoRI & HINDIII

The Last part is a bit confusing. Please help

o 9/25/2018 11:55 PM3109/25/2018 07:17 PM D Gradebook Print Question 16 of 39 The following t abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. table shows where different restriction endonucleases (restriction enzymes) cleave DNA. TMaPd Enzyme Target sequenceCleevege EcoR EcoRV 5 GATATC 3 Haelll Hindlll PpuMI5 RGGWCCY 3 5 GAATTC 3' 3 CTTAAG 5 5 G G 5 5 GAT ATC 3 3' CTA TAG 5 3 CTATAG 5 5 GGCC 3 3' CCGG 5. 'CC GG 5 5' AAGCTT 3 3. TTCGAA 5. 5' A 3. TTCGA AGCTT 3 A5. 5 RG GWCCY3 3. YCCWGGR 5' | 3. YCCWG GR 5 Which restriction endonuclease(s) would cleave a DNA molecule at the following sequences? a) 5 ATCGAACTAGGCC 3 EcoRV EcoR Haell Hindi; b) 5 TCCAAGCTTGAATTO 3 ECOR Hindill Hael ECORV below. Plasmid DNA and a gene of interest are out with the enzyme RpuMi Write a possible sequence of bases for the sticky end of the gene in the 5 to 3 di 5t 3" Hint Previous

Explanation / Answer

Answer: As it can be clearly seen from the information, the site of digestion for enzyme PpuMI remains to be 5' RG' GWCCY 3', thus giving sticky ends after digestion. Here, it is important to note that the nucleotides represented by R, W and Y can vary depending upon the nature of sequence. However, more importantly, the nature of digestion site is fixed an donly a guanine residue is required to be present. Hence, the sticky ends will have following make up in DNA as well as plasmid:

5' RG....GWCCY 3'

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