To determine the cause of the auxotroph in two of your yeast strains, you are go
ID: 150122 • Letter: T
Question
To determine the cause of the auxotroph in two of your yeast strains, you are going to set up a cross, B x C. Why can these strains mate with each other?
A diploid will be formed from the B x C cross. What is the predicted genotype of this diploid?
Which plates allow the diploid to grow, but not the two haploid parents? (The word “complementation” must be in your answer)
Strain
SD+His
SD+His+Ura
SD+His+Leu
SD+His
+Ura+Leu
A
+
+
+
+
+
+
+
+
B
+
+
+
+
-
-
+
+
C
+
+
-
-
+
+
+
+
What do you think will be the genotype of the mating type locus in this diploid?
Strain
SD+His
SD+His+Ura
SD+His+Leu
SD+His
+Ura+Leu
A
+
+
+
+
+
+
+
+
B
+
+
+
+
-
-
+
+
C
+
+
-
-
+
+
+
+
Explanation / Answer
Here B and C will cross between two genotypes SD+His+Ura and SD+His+Leu. These would make diploids because strain B can complement strain C to grow in SD+His+Ura condition; while SD+His+Leu. Plate C will complement Plate B from being executed.
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