4. Questions (to be turned in at the end of lab) I. Proteins are made from amino

Question

4. Questions (to be turned in at the end of lab) I. Proteins are made from amino acids. Why does the biuret reagent only detect protein but not amino acids? Giure 4 2. What kind of sugars does Benedict's reagent detect? What kind of sugars is the Benedict's assay insensitive to? Why? 3. Disaccharides and starches are all made by polymerizing monosacharrides. Why does Lugol's reagent only detect starches but not sucrose? Human nutrition is essentially the study of how to best supply your body's cells with the molecules needed for normal operation. Can you think of any molecules or nutrients that your body needs besides the four major classes of biological macromolecules? 4.

Explanation / Answer

ANS 1) Biuret reagent does not detect amino acids as it responds only when two or more peptides, polypeptides and proteins present.

ANS 2) Benedict's test is utilized to test basic sugars like glucose. It is an unmistakable blue color solution which is a mix of copper sulfate, sodium citrate, and sodium carbonate.Whereas, this test is sensitive for non dissociating sugars as they do not react with them

ANS 3) Lugo detects only starches because this test is a response to the consequence of the development of polyiodide chains when it reacts with starch.

ANS 4) Minerals and Vitamins are also essential among four macronutrients like fats, proteins, carbohydrates and water for our body

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